codon optimization and dna synthesis Search Results


cho-cell codon optimization and dna synthesis  (Twist Bioscience)
90
Twist Bioscience cho-cell codon optimization and dna synthesis
Cho Cell Codon Optimization And Dna Synthesis, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cho-cell codon optimization and dna synthesis/product/Twist Bioscience
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cho-cell codon optimization and dna synthesis - by Bioz Stars, 2026-03
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aptsap vectors dna synthesis and codon optimization for mammalian cell expression  (GenScript corporation)
90
GenScript corporation aptsap vectors dna synthesis and codon optimization for mammalian cell expression
Schematic representation of <t>APTSAP,</t> <t>APTSAPKQ</t> and pBlue_SAP DNA sequences. The nucleotide sequence of AS1411 [5′-d (GGT​GGT​GGT​GGT​TGT​GGT​GGT​GGT​GG)-3] was placed at the 5′ terminus of a synthetic construct containing the CMV promoter, the saporin gene, a chimeric intron derived from pCI vector, and a poly-adenylation signal at the 3′ terminus and inserted into pBluescript II KS (+) vector using a SmaI restriction site. In the APTSAPKQ construct the saporin gene sequence was substituted with the saporin KQ mutated sequence. The pBlue_SAP construct lacks the 5′ AS1411 aptamer sequence. In the scheme we also indicated the PCR primers at their relative positions (FW1/RV1, FW2/RV2, FW3/RV3). This map was created by the SnapGene Viewer freeware software (“SnapGene software” from Insightful Science; available at http://snapgene.com ).
Aptsap Vectors Dna Synthesis And Codon Optimization For Mammalian Cell Expression, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aptsap vectors dna synthesis and codon optimization for mammalian cell expression/product/GenScript corporation
Average 90 stars, based on 1 article reviews
aptsap vectors dna synthesis and codon optimization for mammalian cell expression - by Bioz Stars, 2026-03
90/100 stars
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codon optimization and de novo synthesis of dna  (Epoch Life Signs Inc)
90
Epoch Life Signs Inc codon optimization and de novo synthesis of dna
Schematic representation of <t>APTSAP,</t> <t>APTSAPKQ</t> and pBlue_SAP DNA sequences. The nucleotide sequence of AS1411 [5′-d (GGT​GGT​GGT​GGT​TGT​GGT​GGT​GGT​GG)-3] was placed at the 5′ terminus of a synthetic construct containing the CMV promoter, the saporin gene, a chimeric intron derived from pCI vector, and a poly-adenylation signal at the 3′ terminus and inserted into pBluescript II KS (+) vector using a SmaI restriction site. In the APTSAPKQ construct the saporin gene sequence was substituted with the saporin KQ mutated sequence. The pBlue_SAP construct lacks the 5′ AS1411 aptamer sequence. In the scheme we also indicated the PCR primers at their relative positions (FW1/RV1, FW2/RV2, FW3/RV3). This map was created by the SnapGene Viewer freeware software (“SnapGene software” from Insightful Science; available at http://snapgene.com ).
Codon Optimization And De Novo Synthesis Of Dna, supplied by Epoch Life Signs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/codon optimization and de novo synthesis of dna/product/Epoch Life Signs Inc
Average 90 stars, based on 1 article reviews
codon optimization and de novo synthesis of dna - by Bioz Stars, 2026-03
90/100 stars
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codon optimization and dna synthesis  (Gene Universal Inc)
90
Gene Universal Inc codon optimization and dna synthesis
Schematic representation of <t>APTSAP,</t> <t>APTSAPKQ</t> and pBlue_SAP DNA sequences. The nucleotide sequence of AS1411 [5′-d (GGT​GGT​GGT​GGT​TGT​GGT​GGT​GGT​GG)-3] was placed at the 5′ terminus of a synthetic construct containing the CMV promoter, the saporin gene, a chimeric intron derived from pCI vector, and a poly-adenylation signal at the 3′ terminus and inserted into pBluescript II KS (+) vector using a SmaI restriction site. In the APTSAPKQ construct the saporin gene sequence was substituted with the saporin KQ mutated sequence. The pBlue_SAP construct lacks the 5′ AS1411 aptamer sequence. In the scheme we also indicated the PCR primers at their relative positions (FW1/RV1, FW2/RV2, FW3/RV3). This map was created by the SnapGene Viewer freeware software (“SnapGene software” from Insightful Science; available at http://snapgene.com ).
Codon Optimization And Dna Synthesis, supplied by Gene Universal Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/codon optimization and dna synthesis/product/Gene Universal Inc
Average 90 stars, based on 1 article reviews
codon optimization and dna synthesis - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Schematic representation of APTSAP, APTSAPKQ and pBlue_SAP DNA sequences. The nucleotide sequence of AS1411 [5′-d (GGT​GGT​GGT​GGT​TGT​GGT​GGT​GGT​GG)-3] was placed at the 5′ terminus of a synthetic construct containing the CMV promoter, the saporin gene, a chimeric intron derived from pCI vector, and a poly-adenylation signal at the 3′ terminus and inserted into pBluescript II KS (+) vector using a SmaI restriction site. In the APTSAPKQ construct the saporin gene sequence was substituted with the saporin KQ mutated sequence. The pBlue_SAP construct lacks the 5′ AS1411 aptamer sequence. In the scheme we also indicated the PCR primers at their relative positions (FW1/RV1, FW2/RV2, FW3/RV3). This map was created by the SnapGene Viewer freeware software (“SnapGene software” from Insightful Science; available at http://snapgene.com ).

Journal: Frontiers in Pharmacology

Article Title: Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

doi: 10.3389/fphar.2021.588306

Figure Lengend Snippet: Schematic representation of APTSAP, APTSAPKQ and pBlue_SAP DNA sequences. The nucleotide sequence of AS1411 [5′-d (GGT​GGT​GGT​GGT​TGT​GGT​GGT​GGT​GG)-3] was placed at the 5′ terminus of a synthetic construct containing the CMV promoter, the saporin gene, a chimeric intron derived from pCI vector, and a poly-adenylation signal at the 3′ terminus and inserted into pBluescript II KS (+) vector using a SmaI restriction site. In the APTSAPKQ construct the saporin gene sequence was substituted with the saporin KQ mutated sequence. The pBlue_SAP construct lacks the 5′ AS1411 aptamer sequence. In the scheme we also indicated the PCR primers at their relative positions (FW1/RV1, FW2/RV2, FW3/RV3). This map was created by the SnapGene Viewer freeware software (“SnapGene software” from Insightful Science; available at http://snapgene.com ).

Article Snippet: APTSAP, APTSAPKQ and pBlue_SAP vectors DNA synthesis and codon optimization for mammalian cell expression were from GenScript (United States), while the single strands corresponding to AS1411 (ssAPT) was purchased from Eurofins Genomics (Germany).

Techniques: Sequencing, Construct, Derivative Assay, Plasmid Preparation, Software

Dose-response curves showing respectively the effects of ssAPT, APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) on U87MG cells. Cells (1.6 × 10 3 /well) were seeded in 96-well plates in a final volume of 200 µl of medium (5% FBS) containing the appropriate concentrations (0, 0.001, 0.01, 1, 10, 50 μmol/L) of ssAPT or the appropriate concentrations (0, 0.001, 0.01, 0.02, 0.05 and 0.1 μmol/L) of APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) for 96 h. All the plasmids were denatured at 95°C for 2 min in 10 mM Tris/HCl buffer pH 7.5 containing 100 mM NaCl and 2.5 mM KCl immediately before use. Cell viability was examined using a MTS assay and expressed as percentage of control untreated cells. ssAPT IC 50 : 4.30 × 10 −6 ± 1.70 × 10 −6 M (mean ± S.E.M.); APTSAP IC 50 : 1.30 × 10 −8 ± 2.42×10 −9 M (mean ± S.E.M.). The data reported are representative of a set of at least three independent experiments and are reported as mean ± SD.

Journal: Frontiers in Pharmacology

Article Title: Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

doi: 10.3389/fphar.2021.588306

Figure Lengend Snippet: Dose-response curves showing respectively the effects of ssAPT, APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) on U87MG cells. Cells (1.6 × 10 3 /well) were seeded in 96-well plates in a final volume of 200 µl of medium (5% FBS) containing the appropriate concentrations (0, 0.001, 0.01, 1, 10, 50 μmol/L) of ssAPT or the appropriate concentrations (0, 0.001, 0.01, 0.02, 0.05 and 0.1 μmol/L) of APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) for 96 h. All the plasmids were denatured at 95°C for 2 min in 10 mM Tris/HCl buffer pH 7.5 containing 100 mM NaCl and 2.5 mM KCl immediately before use. Cell viability was examined using a MTS assay and expressed as percentage of control untreated cells. ssAPT IC 50 : 4.30 × 10 −6 ± 1.70 × 10 −6 M (mean ± S.E.M.); APTSAP IC 50 : 1.30 × 10 −8 ± 2.42×10 −9 M (mean ± S.E.M.). The data reported are representative of a set of at least three independent experiments and are reported as mean ± SD.

Article Snippet: APTSAP, APTSAPKQ and pBlue_SAP vectors DNA synthesis and codon optimization for mammalian cell expression were from GenScript (United States), while the single strands corresponding to AS1411 (ssAPT) was purchased from Eurofins Genomics (Germany).

Techniques: MTS Assay, Control

Representative images observed with an optical microscope of U87 cells after 96h of treatment. Controls (A) ; ssAPT 25 µM (B) ; APTSAP 20 nM (C) ; APTSAPKQ 20 nM (D) ; pBlue_SAP 20 nM (E) .

Journal: Frontiers in Pharmacology

Article Title: Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

doi: 10.3389/fphar.2021.588306

Figure Lengend Snippet: Representative images observed with an optical microscope of U87 cells after 96h of treatment. Controls (A) ; ssAPT 25 µM (B) ; APTSAP 20 nM (C) ; APTSAPKQ 20 nM (D) ; pBlue_SAP 20 nM (E) .

Article Snippet: APTSAP, APTSAPKQ and pBlue_SAP vectors DNA synthesis and codon optimization for mammalian cell expression were from GenScript (United States), while the single strands corresponding to AS1411 (ssAPT) was purchased from Eurofins Genomics (Germany).

Techniques: Microscopy

Dose-response curves showing respectively the effect of ssAPT, APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) on control NIH3T3 cells. Cells (1.6 × 10 3 /well) were seeded in 96-well plates in a final volume of 200 ul of medium (5% FBS) containing appropriate concentrations (0, 0.001, 0.01, 1, 10, 50 μmol/L) of ssAPT and appropriate concentrations (0, 0.001, 0.01, 0.02, 0.05 and 0.1 μmol/L) of APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) and for 96 h. All the plasmids were denatured at 95°C for 2 min in 10 mM Tris/HCl buffer pH 7.5 containing 100 mM NaCl and 2.5 mM KCl immediately before use. Cell viability was examined using a MTS assay and expressed as percentage of control untreated cell. The data presented are representative of a set of at least three independent experiments and are reported as mean ± SD.

Journal: Frontiers in Pharmacology

Article Title: Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

doi: 10.3389/fphar.2021.588306

Figure Lengend Snippet: Dose-response curves showing respectively the effect of ssAPT, APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) on control NIH3T3 cells. Cells (1.6 × 10 3 /well) were seeded in 96-well plates in a final volume of 200 ul of medium (5% FBS) containing appropriate concentrations (0, 0.001, 0.01, 1, 10, 50 μmol/L) of ssAPT and appropriate concentrations (0, 0.001, 0.01, 0.02, 0.05 and 0.1 μmol/L) of APTSAP, APTSAPKQ, pBlue_SAP and pBluescript II KS (+) and for 96 h. All the plasmids were denatured at 95°C for 2 min in 10 mM Tris/HCl buffer pH 7.5 containing 100 mM NaCl and 2.5 mM KCl immediately before use. Cell viability was examined using a MTS assay and expressed as percentage of control untreated cell. The data presented are representative of a set of at least three independent experiments and are reported as mean ± SD.

Article Snippet: APTSAP, APTSAPKQ and pBlue_SAP vectors DNA synthesis and codon optimization for mammalian cell expression were from GenScript (United States), while the single strands corresponding to AS1411 (ssAPT) was purchased from Eurofins Genomics (Germany).

Techniques: Control, MTS Assay

Representative images observed with an optical microscope of NIH3T3 cells after 96 h of treatment. Controls (A) ; ssAPT 25 µM (B) ; APTSAP 20 nM (C) ; APTSAPKQ 20 nM (D) ; pBlue_SAP 20 nM (E) .

Journal: Frontiers in Pharmacology

Article Title: Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

doi: 10.3389/fphar.2021.588306

Figure Lengend Snippet: Representative images observed with an optical microscope of NIH3T3 cells after 96 h of treatment. Controls (A) ; ssAPT 25 µM (B) ; APTSAP 20 nM (C) ; APTSAPKQ 20 nM (D) ; pBlue_SAP 20 nM (E) .

Article Snippet: APTSAP, APTSAPKQ and pBlue_SAP vectors DNA synthesis and codon optimization for mammalian cell expression were from GenScript (United States), while the single strands corresponding to AS1411 (ssAPT) was purchased from Eurofins Genomics (Germany).

Techniques: Microscopy

U87 (A) and 3T3 cells (B) were treated with 20 nM APTSAP for 96 h, total RNA was extracted from cell pellets. PCR was performed on the corresponding cDNA using the three couples of primers, as described in the Materials and Methods section. pET11d plasmid carrying the wild type saporin gene (pET11d-SAP) was used as a positive control. Amplified products are indicated by arrows, and the expected dimensions are: FW1/RV1:765 bp, FW2/RV2: 204 bp, FW3/RV3: 621bp. Lanes 1-3-5: pET11d-SAP; lanes 2-4-6: treated.

Journal: Frontiers in Pharmacology

Article Title: Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

doi: 10.3389/fphar.2021.588306

Figure Lengend Snippet: U87 (A) and 3T3 cells (B) were treated with 20 nM APTSAP for 96 h, total RNA was extracted from cell pellets. PCR was performed on the corresponding cDNA using the three couples of primers, as described in the Materials and Methods section. pET11d plasmid carrying the wild type saporin gene (pET11d-SAP) was used as a positive control. Amplified products are indicated by arrows, and the expected dimensions are: FW1/RV1:765 bp, FW2/RV2: 204 bp, FW3/RV3: 621bp. Lanes 1-3-5: pET11d-SAP; lanes 2-4-6: treated.

Article Snippet: APTSAP, APTSAPKQ and pBlue_SAP vectors DNA synthesis and codon optimization for mammalian cell expression were from GenScript (United States), while the single strands corresponding to AS1411 (ssAPT) was purchased from Eurofins Genomics (Germany).

Techniques: Plasmid Preparation, Positive Control, Amplification